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Image Search Results
Journal: Redox Report : Communications in Free Radical Research
Article Title: LncRNA Fendrr: involvement in the protective role of nucleolin against H 2 O 2 -induced injury in cardiomyocytes
doi: 10.1080/13510002.2023.2168626
Figure Lengend Snippet: lncRNAs regulated by nucleolin in cardiomyocytes. (A) Heatmap profile of lncRNA microarray analysis. Green to red colors indicate low to high transcriptional levels. The lncRNAs differentially expressed between the two groups were identified through paired t-test P ≤ 0.05 and a fold change (FC) ≥ 2.5; n = 3 independent biological samples for each group. (B) The expression of 15 selected lncRNAs in chip test, where 10 differentially expressed up-regulated lncRNAs (up) and 5 differentially expressed down-regulated lncRNAs (down) were selected. (C) The interactions between nucleolin and lncRNAs were confirmed by RIP and identified via qRT-PCR. *, P < 0.05, vs. IgG group, n = 3. pcDNA3.1, the empty vector served as negative control; pcDNA3.1-Nuc, overexpression nucleolin group. (D) Bioinformatics website predicted Fendrr binding elements with nucleolin.
Article Snippet: Using the Aglient
Techniques: Microarray, Expressing, Quantitative RT-PCR, Plasmid Preparation, Negative Control, Over Expression, Binding Assay